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. 2020 Feb 21;133(4):jcs239616. doi: 10.1242/jcs.239616

Fig. 1.

Fig. 1.

Cep44 is a proximal end centriolar protein whose level changes during the cell cycle. (A) U2OS cells or RPE-1 G0 cells were stained with the indicated antibodies with or without DAPI. GT335, glutamylated tubulin. Scale bar: 10 μm. (B) U2OS cells were stained with the indicated antibodies and images were acquired with 3D-SIM. M, mother centriole; D, daughter centriole. Scale bar: 200 µm. (C) RPE-1 G0 cells expressing GFP–Cep44 were stained with the indicated antibodies. Scale bar: 10 μm. (D) RPE-1 cells were stained with DAPI and the indicated antibodies. Scale bar: 10 μm. (E) Quantification of Cep44 fluorescence intensity at the centrosome. Results are mean±s.e.m. based on three independent experiments with 20 cells per experiment (n=60). **P<0.01 (one-way ANOVA). (F) RPE-1 cell lysates were western blotted with antibodies against Cep44, C-Nap1 or rootletin. α-tubulin was used as loading control. AS, asynchronous. A representative experiment is shown. (G) Quantification of Cep44 level relative to α-tubulin. Results are mean±s.e.m. based on five independent experiments (n=5). *P<0.05 (one-way ANOVA).