Fig. 2.

Cep44 is required for centrosome cohesion. (A) U2OS cells transfected with non-specific (NS) or Cep44 siRNAs (oligo 1 or 2) were stained with the indicated antibodies. Scale bar: 10 µm. (B) The percentage of U2OS cells with separated γ-tubulin dots is presented. (C) The percentage of GFP-positive U2OS cells with separated γ-tubulin dots is presented. (D) FACS analysis of the cell cycle states of U2OS cells transfected with NS or Cep44 siRNAs. For B,C, three independent experiments were performed, and each experiment involved counting >100 cells for each condition.