Fig. 3.

Cep44 is required for the localization of rootletin to the centrosome. (A,B) Quantification of the distance between two centrin dots in U2OS or RPE-1 cells. G1 cells with the best knockdown were chosen for quantification. (C,D) Quantification of rootletin fluorescence intensity at the centrosome in U2OS or RPE-1 cells. (E,F) Quantification of rootletin fluorescence intensity at the centrosome in U2OS or RPE-1 cells. GFP-positive cells were chosen for quantification. (G,H) Quantification of Cep68 fluorescence intensity at the centrosome in U2OS or RPE-1 cells. For A–H, results are mean+s.e.m. based on three independent experiments with 20 cells per experiment (n=60). **P<0.01 (two-tailed Student's t-test).