Figure 1.

miR-25 Is Enriched in the Early Stage of CM Differentiation

(A) Schematic of chemically defined CM differentiation in vitro. (B) The morphology of cells during CM differentiation. (C) Thirty-day-old CMs showed regular sarcomeric structures, as illustrated by immunofluorescent staining of α-ACTININ (green) and TNNT2 (red). (D and E) Cell proliferation decreased from days 4 to 30 as revealed by EdU staining (D). EdU incorporation was quantified using ImageJ software (E). Approximately 2,000 cells were counted in each group. (F) The top 15 most abundant miRNAs at day 6 and their expression levels at day 18 were analyzed by miRNA-seq during hESC differentiation.