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. 2020 Jan 21;19:1299–1308. doi: 10.1016/j.omtn.2020.01.013

Figure 2.

Figure 2

miR-25 Promotes hESC-CM Proliferation

(A) Schematic of the experimental design. (B) qRT-PCR analysis showed that miR-25 expression was significantly increased in hESC-CMs transfected with miR-25 mimics (n = 3) NC = cells transfected with normal control mimics. (C) qRT-PCR analysis showed that the expression of proliferating cell nuclear antigen (PCNA) increased in hESC-CMs transfected with miR-25 mimics (n = 3). NC = cells transfected with normal control mimics. (D) hESC-CMs transfected with miR-25 mimics or NC were stained for sarcomeric α-ACTININ (red) and DAPI (blue). (E) Relative numbers of hESC-CMs treated with miRNA-25 or NC. (n = 3) (F) hESC-CMs transfected with miR-25 mimics or NC were stained with EdU (green), an antibody against α-ACTININ (red) and DAPI (blue). (F) Percentage of Edu+ hESC-CMs treated with miR-25 mimics or NC. Approximately 2,000 cells were counted in each group. (H) hESC-CMs transfected with miR-25 mimics or NC were stained with an antibody against Ki-67 (green), α-ACTININ (red) and DAPI (blue). (I) Percentage of Ki-67+ hESC-CMs treated with miR-25 mimics or NC. Approximately 2,000 cells were counted in each group. Statistical significance was calculated using Student's t test for paired samples. Data are shown as the mean ± SEM. *p < 0.05, ***p < 0.001, ****p < 0.0001.