Figure 5.

miR-25 Promotes CM Proliferation in Zebrafish

(A) Morphology of zebrafish hearts injected with miR-25 mimics or NC. Transgenic zebrafish with myocardium-specific GFP expression were used. (B) Histological section of the cross-sectional areas of miR-25 or NC injection. (C) The cross-sectional areas of miR-25 or NC injection. (n > 6) (D) The confocal picture of single CM injected with miR-25 or NC. (E) The volume of single CM treated with miR-25 or NC. (F) CM of zebrafish hearts injected with miR-25 or NC. (G) The number of CMs after miR-25 or NC injections. A transgenic zebrafish line with myocardium-specific RFP expression in the nuclei was employed. (H) miR-25 injection did not influence heart rate. Statistical significance was calculated using Student’s t test for paired samples. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.