Figure 6.

FBXW7 Is a Direct Target Gene of miR-25 in Zebrafish

(A) The sequence of miR-25 sequences is conserved in human and zebrafish. (B) A potential target site (highlighted in red) of miR-25 on the zebrafish fbxw7 3′ UTR was predicted by TargetScan. (C) Luciferase reporter assay showed that the predicted binding sequence was required for miR-25 inhibition. (D) mCherry sensors were co-injected with EGFP control as indicated. miR-25-duplex injection reduced the mCherry levels in mCherry-fbxw7-3′ UTR, whereas EGFP levels were unchanged. In the mutated sensor, no reduction in mCherry was noted. Experiments were repeated three times; for each group, six embryos were analyzed. Mut, mutant. Statistical significance was calculated using Student’s t test for paired samples. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.