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. 2020 Feb 20;7(1):ENEURO.0388-19.2020. doi: 10.1523/ENEURO.0388-19.2020

Figure 3.

Figure 3.

α-motor neuron loss and NMJ denervation are slightly more pronounced in female SOD1G37R/YFP mice at P360. A, Representative image of lumbar spinal cord sections from a female WT/YFP mice at P360 (red: ChAT; blue: NeuN; green: YFP). B, Representative image of NMJs in the tibialis anterior muscle of a female WT/YFP mice at P360 (red: α-BTX; green: NF-M and SV2). C, Representative examples of lumbar spinal cord sections from female (top) and male (bottom) SOD1G37R/YFP mice at P360. D, Representative images of NMJs in the tibialis anterior muscle of female (top) and male (bottom) SOD1G37R/YFP mice at P360. E, F, Quantification of the number of α-motor neurons (E, ChAT+ NeuN+ cells) and γ-motor neurons (F, ChAT+ NeuN– cells) per ventral horn at P360 (WT female: N = 4; WT male: N = 2; SOD1 female: N = 4; SOD1 male: N = 5). G, Quantification of the percentage of fully innervated NMJs in the tibialis anterior at P360 (WT female: N = 4, n = 1078; WT male: N = 2, n = 446; SOD1 female: N = 4, n = 977; SOD1 male: N = 5, n = 820). Data are presented as mean ± SEM. Main effects and interactions are reported in Table 2 and in the text. Asterisks represent biologically relevant statistically significant differences in the post hoc test (E, F, Tukey’s multiple comparisons; G, Holm–Sidak’s correction); *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar: 100 μm.