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Lower Limit of Detection Testing
M. arginini or M. hominis gDNA was diluted to the indicated concentrations in EB and amplified with universal Mycoplasma spp. primers. M. arginini, 150 or 15 GC/200 μL; M. hominis, 1,000 or 100 GC/200 μL. Lane L, ladder; lane N, PCR negative control. Expected Mycoplasma-specific band size was 400–500 bp. Representative gels from two rounds of testing are shown.
