Figure 2.

Ion chromatography-mass spectrometry (IC-MS) analysis of glutamate and other Krebs cycle metabolites derived from labeled Gln showed enhanced glutaminolysis but inhibition of gluconeogenesis in the livers of miR-122 KO mice. The polar extracts from the livers were analyzed by IC-MS. Elevated levels (A) and fractional distribution (B) of selected and total labeled isotopologues (Cx or CxNx; x ≥ 1) of (I) Glu, (II) α-ketoglutarate, (III) citrate, and (IV) isocitrate were evident in miR-122 KO mouse livers compared with control mouse livers. Also noted were the decreased levels with little changes in the fractional enrichment of ¹³C₃- (C3) and total labeled isotopologues (Cx) of glucose-6-phosphate (G6P) (V) and frutose-6-phosphate (F6P) (VI) in miR122 LKO/KO mouse livers compared with control mouse livers. * (gray), *, and *** denote Q values of ≤0.1, 0.05, and 0.005, respectively, with n = 3–4.