Primer sequences for cloning into psi-CHECK2 vector

mmu-Gls-3′-UTR-F: 5′ CCGCTCGAGTTAGCTTTAGTTTACACATA (Forward primer with XhoI site)

mmu-Gls-3′-UTR-R: 5′ ATAAGAATGCGGCCGCTCTTTTTCTGAGCAACTAAC (Reverse oligo with Not I site)

mmu-Gls-Del-F: 5′ATCTCGAGATGCTTCTGTTTATTTTTAAA (Forward primer for deletion of miR122 binding site in Gls 3′-UTR)

mmu-Gls-Del-R: 5′ ATGCGGCCGCCCTTTTCTTAATATCAGAGTT (Reverse primer for deletion of miR122 binding site)

mmu-Slc1a5-F: 5′ AGC TTT GTT TAA ACT GGA GAA ATG GAC TGG ATG A (Forward primer with PMEI site)

mmu-Slc1a5-R: 5′ ATA AGA ATG CGG CCG CTT TGA CAC CAT TTT ATT GCT (Reverse primer with Not I RE site)

mmu-Slc1a5-Del1: 5′ AGC TTT GTT TAA ACG GAA TCT AGG GGT CGG TGT C (Forward primer for deletion of site 1)

mmu-Slc1a5-Mut2-F: 5′ ATG ACA GAA ATG TGA GGC ATG AGT CTC G (Forward primer for mutation of site 2)

mmu-Slc1a5-Mut2-R: 5′ AGC TTT GTT TAA ACT GGA GAA ATG GAC TGG ATG A (Reverse primer for mutation of site 2)

mmu-Slc1a5-Del3: 5′ ATA AGA ATG CGG CCG CAC ATT TAT TCT TGG CTT TCC (Reverse primer for deletion of site 3)