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Potential lysosome-targeting CL Probe detects PMA generated ONOO− in murine macrophages. RAW 264.7 macrophages were stimulated with PMA (1 μg/ml) for 1 h. CL (20 μM), was incubated for 30 min. Blue channel: λex = 405 nm, λem = 420–460 nm. Scale bar: 20 μm. Magnification x60. n = 3.
