Figure 2. APOE effects astrocyte glucose uptake.
A) E2 astrocytes take up more glucose, while E4 astrocytes take up less glucose, relative to E3. Immortalized astrocytes expressing human APOE were treated with 3H-2-deoxyglucose (5.6uCi/mL) in vitro and uptake was measured by a scintillation proximity assay (SPA) over 120 minutes. Total area under the curve (AUC) was determined. Values represent mean +/− SEM (n=3). Data analyzed by two-way ANOVA of repeated measures (SPA) or t-test (AUC). *** p < 0.001.