Table 1.
qPCR primer sequences
| Gene | Gene Bank | Amplicon size | Sequence 5′-3′ | |
|---|---|---|---|---|
| Reference genes | OAZ1 | NM_008753 | 51 | Fwd AGCGAGAGTTCTAGGGTTGCC |
| Rev CCCCGGACCCAGGTTACTAC | ||||
| L27A | BC086939 | 101 | Fwd TGTTGGAGGTGCCTGTGTTCT | |
| Rev CATGCAGACAAGGAAGGATGC | ||||
| HPRT | NM_013556.2 | 150 | Fwd ACAGGCCAGACTTTGTTGGA | |
| Rev ACTTGCGCTCATCTTAGGCT | ||||
| Phagocytosis receptors | P2Y12 | NM_027571 | 88 | Fwd GCAGAACCAGGACCATGGAT |
| Rev CTGACGCACAGGGTGCTG | ||||
| MerTK | NM_008587.1 | 131 | Fwd AAGGTCCCCGTCTGTCCTAA | |
| Rev GCGGGGAGGGGATTACTTTG | ||||
| Axl | NM_009465.4 | 86 | Fwd GTTGGTGTCTGGAGGATGGG | |
| Rev TGTGTGTCCTTATGGGCTGC | ||||
| Peptides and hormones | VGF | NM_001039385.1 | 74 | Fwd CACCGGCTGTCTCTGGC |
| Rev AAGGAAGCAGAAGAGGACGG | ||||
| Cartpt | NM_013732.7 | 106 | Fwd GCGCTATGTTGCAGATCGAAG | |
| Rev GCGTCACACATGGGGACTTG | ||||
| FGF2 | NM_008006.2 | 113 | Fwd CGGCTGCTGGCTTCTAAGTG | |
| Rev AGTGCCACATACCAACTGGAG | ||||
| Trophic factors | VEGFA | NM_001025250.3 | 88 | Fwd GGCCTCCGAAACCATGAACT |
| Rev CTGGGACCACTTGGCATGG | ||||
| PDGFα | NM_008808.3 | 94 | Fwd TACCCCGGGAGTTGATCGAG | |
| Rev TCAGCCCCTACGGAGTCTATC | ||||
| IGF-1 | NM_010512.4 | 122 | Fwd TTACTTCAACAAGCCCACAGG | |
| NM_184052.3 | Rev GTGGGGCACAGTACATCTCC | |||
| NM_001111274.1 | ||||
| NM_001111275.1 | ||||
| NM_001111276.1 | ||||
| EGF | NM_010113.3 | 136 | Fwd GGACTGAGTTGCCCTGACTC | |
| Rev CAATATGCATGCACACGCCA | ||||
| GDNF | NM_010275.2 | 145 | Fwd CGCTGACCAGTGACTCCAA | |
| Rev TGCCGATTCCTCTCTCTTCG | ||||
| Matrix protein | Mmp3 | NM_010809.2 | 88 | Fwd ACCCAGTCTACAAGTCCTCCA |
| Rev GGAGTTCCATAGAGGGACTGA | ||||
| Surface ligands | Jag1 | NM_013822.5 | 119 | Fwd TTCAGGGCGATCTTGCATCA |
| Rev CACACCAGACCTTGGAGCAG | ||||
| Dll4 | NM_019454.3 | 113 | Fwd GGTTACACAGTGAGAAGCCAGA | |
| Rev GGCAATCACACACTCGTTCC | ||||
| Cytokines | Csf3 | NM_009971.1 | 70 | Fwd GCAGCCCAGATCACCCAGAAT |
| Rev TGCAGGGCCATTAGCTTCAT | ||||
| IL1β | NM_000576.2 | 72 | Fwd AGATGAAGTGCTCCTTCCAGG | |
| Rev GGTCGGAGATTCGTAGCTGG | ||||
| IL6 | NM_000600.3 | 107 | Fwd GAAAGCAGCAAAGAGGCACTG | |
| Rev TTCACCAGGCAAGTCTCCTCAT | ||||
| TNFα | NM_000594.3 | 142 | Fwd TGCACTTTGGAGTGATCGGC | |
| Rev GCTTGAGGGTTTGCTACAACA | ||||
| TGFβ | NM_000660.5 | 112 | Fwd TCCTGGCGATACCTCAGCAA | |
| Rev CAATTTCCCCTCCACGGCTC |
List of primers used to amplify reference genes, phagocytosis receptors, peptides and hormones, trophic factors, matrix protein, surface ligands, and cytokines. The gene name, Gene Bank accession number, amplicon size, and sequence are listed.