Figure 7.

P2Y1 and P2Y13 immunostaining. A, Immunostaining against P2Y1 and P2Y13 receptors in an IBA1⁺ microglia (amoeboid cell of PTC). B, P2Y1 and P2Y13 receptor immunostaining for a ramified PTC cell. A, B, From left, Iba1 staining (green), P2Y1 staining (blue), P2Y13 staining (red), and merge of P2Y1, P2Y13, and Iba1 staining. Arrows indicate the spatial overlap of P2Y1 and P2Y13 staining in Iba1⁺ cells in both A and B. Such overlap was seen in 47% (45 of 95) of Iba1⁺ microglia. C, Ramified cells (n = 47) were more likely than amoeboid cells (n = 48) to be immunopositive for P2Y1 (blue) or P2Y13 receptors (red).