Figure 2.

CREB family transcription factors are the main regulators of BDNF transcriptional autoregulation. Primary neurons were transduced at 2 DIV with AAV particles coding for indicated dominant-negative transcription factors or EGFP. At 8 DIV, neurons were treated with 50 ng/ml BDNF for the indicated time periods, and the expression levels of total BDNF mRNA (BDNF coding) were measured using qRT-PCR. The expression level of respective transcripts in cells transduced with EGFP-encoding AAV particles and not treated with BDNF was set as 1. Average of three independent experiments (n = 3) is shown. Error bars indicate SEM. Statistical significance between the respective transcript levels in EGFP and respective dominant-negative-overexpressing cells at respective time points: **p < 0.01; *p < 0.05 (paired two-tailed t test, corrected for multiple comparisons using Holm–Sidak method).