Figure 5.

Early, but not late, phase of BDNF transcriptional autoregulation requires CREB-CBP/p300 interaction. At 8 DIV, primary neurons were treated with 5 μm CBP-CREB interaction inhibitor (CCII) or with DMSO as a control for 1 h before treating the cells with 50 ng/ml BDNF for the indicated time periods. qRT-PCR was used to measure c-Fos, total BDNF (BDNF coding), and BDNF transcript levels. The expression level of respective transcripts in vehicle-treated cells not treated with BDNF was set as 1. Average of three independent experiments (n = 3) is shown. Error bars indicate SEM. Statistical significance between respective transcript levels in DMSO and CCII-treated cells at respective time points: **p < 0.01; *p < 0.05 (paired two-tailed t test, corrected for multiple comparisons using Holm–Sidak method).