Table 1.
Authentication of primary antibodies used in this study
| Antigen | Source | Host | Catalog no. (clone) | Dilution used | Application | Authentication | Antibody ID | References (PMID) |
|---|---|---|---|---|---|---|---|---|
| Atoh7 | Novus | Rabbit | NBP1-88639 | 1:500 | IF | 1, 2, 3, 4 | AB_11034390 | 29225067 |
| Lot R12065 | ||||||||
| Blimp1/Prdm1 | Santa Cruz | Rat | SC-47732 (6D3) | 1:100 | IF | 1, 3 | AB_628168 | 30184502 |
| 30463012 | ||||||||
| 30850343 | ||||||||
| Arr3 | EMD Millipore | Rabbit | AB15282 | 1:1000 | IF | 1, 3 | AB_11210270 | 26453550 |
| Cone arrestin | 25798616 | |||||||
| 24651551 | ||||||||
| cleaved PARP (Rodent-specific) | Cell Signaling | Rabbit | 94885 | 1:500 | IF | 1, 3 | AB_2800237 | this paper |
| RFP-Biotin | Abcam | Rabbit | AB34771 | 1:1000 | IF | 1, 3 | AB_777699 | 26587807 |
| 28641110 | ||||||||
| 28817801 | ||||||||
| GFP | Aves | Chicken | GFP-1020 | 1:3000 | IF | 1, 3 | AB_10000240 | >100 in registry |
| Glutamine Synthetase | EMD Millipore | Mouse | MAB302 (GS-6) | 1:1000 | IF | 1, 3 | AB_309678 | 26283925 |
| 26527153 | ||||||||
| 25907681 | ||||||||
| Hes1 | Cell Signaling | Rabbit | 11988 D6P2U | 1:500 | IF | 1, 2, 3, 4 | AB_2728766 | 29681454 |
| 30059908 | ||||||||
| Hes1 | Brown lab | Rabbit | N/A | 1:500 | IF | 2, 3, 4 | 30059908 | |
| 28675662 | ||||||||
| 25100656 | ||||||||
| 19828801 | ||||||||
| 16038893 | ||||||||
| Mitf | Thermo Fisher | Mouse (IgG1) | MS-771-P1 (C5) | 1:100 | IF | 1, 3 | AB_141542 | this paper |
| Otx2-Biotin | R&D Systems | Goat | BAF1979 | 1:1000 | IF | 1, 3 | AB_2157171 | 28089909 |
| 30048641 | ||||||||
| Pou4f/Brn3 * | Santa Cruz | Goat | SC 6026 | 1:50 | IF | 1, 3 | AB_673441 | 18626943 |
| 21246546 | ||||||||
| 21452196 | ||||||||
| +12 more | ||||||||
| Pax2 | BioLegend Covance | Rabbit | 901001 | 1:1000 | IF | 1, 2, 3 | AB_2565001 | 20437530 |
| PRB-276P | AB_291611 | 20737600 | ||||||
| 22473852 | ||||||||
| 30659795 | ||||||||
| Pax6 | BioLegend Covance | Rabbit | 901301 | 1:1000 | IF | 1, 2, 3, 4 | AB_2565003 | 28132835 |
| PRB-278P | AB_291612 | +24 more | ||||||
| Pax6 | Santa Cruz | Mouse (IgG1) | SC-32766 (AD2.38) | 1:50 | IF | 1, 2, 3 | AB_628107 | >40 in Registry |
| Rax | TaKaRa | Rabbit | M228 | 1:1000 | IF | 3 | 29117559 | |
| this paper | ||||||||
| Sox9 | Millipore | Rabbit | AB5535 | 1:500 | IF | 1, 3 | AB2239761 | 18626943 |
| + 33 others | ||||||||
| Thrb2 | Douglas Forrest, NIH | Rabbit | N/A | 1:3000 | IF | 2, 3 | 19282790 | |
| Tubb3 | BioLegend | Rabbit | 802001 | 1:1000 | IF | 1, 3 | AB_2564645 | 29247817 |
| Covance | PRB-435P | AB_291637 | 16874803 | |||||
| +others | ||||||||
| Tubb3 | BioLegend | Mouse | MMS-435-P | 1:50 | IF | 1, 3 | AB_2313773 | 16680766 |
| 16786555 | ||||||||
| +160 more | ||||||||
| Vsx2/Chx10 | Exalpha | Sheep | X1180P | 1:500 | IF | 1, 3 | AB_2314191 | 19827163 |
| N-terminus | 27565351 |
Authentication methods used were as follows: 1, Antibody identification at http://antibodyregistry.org; 2, Genetic validation; 3, Comparison with known published patterns in the literature; and 4, Colabeling of tagged protein with endogenous protein.
*Discontinued.
IF, Immunofluorescence.