FIG. 4.

Fabrication of SAPNS microspheres using a microfluidic droplet generation method. (A) Schematic of the microsphere fabrication process. (B) Quantification of microsphere diameter versus flow rate ratio; mean ± SEM; (C) Immunocytochemical staining of cells encapsulated within SAPNS microspheres displaying the following markers: Tuj1 (β-III tubulin): neuron, MAP2: mature neuron, TH: DA neuron, FOXA2: midbrain DA neuron, PITX3: midbrain DA neuron, En-1 (Engrailed-1): midbrain DA neuron, DAPI: nucleus. Scale bar: 50 μm. Color images are available online.