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Progression of cardiac fibrosis. Percentage of picrosirius red stained collagen fibers (A,D) and gene expression of Ctgf (B) and Tgf-β (C) increased with the progression of diabetes. Data are presented as mean ± SEM. *P < 0.05 vs age-matched non-diabetic mice (two-way ANOVA with Benjamini and Hochberg post hoc test). Representative images for each endpoint are shown (D). Scale bar = 40 μm. n = 7–11/group (refer to Supplementary Figure S1).
