Figure 1.

Strategy for generating the modified MSP1 locus. Modification of the MSP1 locus in P. berghei ANKA (PbANKA) (A) and in P. berghei NK65 (PbNK65) (B) for the generation of the Pb/Pv${\text{MSP1}}_{19}^{\text{ANKA}}$ and of the Pb/PvMSP1₁₉GFP^NK65 hybrid parasites, respectively. Arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3′UTR of msp1; black circle lollipop, trap 3′UTR; black box, hDHFR selection marker cassette; green box, GFP cassette. P. berghei sequences are in gray and P. vivax sequences in orange.