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. 2020 Feb 19;11:28. doi: 10.3389/fimmu.2020.00028

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Copyright © 2020 Dobrescu, de Camargo, Gimenez, Murillo, Amorim, Marinho, Soares, Boscardin and Bargieri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sera from mice immunized with HIS₆-FliC-PvMSP1₁₉-PADRE + poly (I:C) and challenged inhibit merozoite invasion and has increased antibody affinity to PvMSP1₁₉. Inhibition of Pb/PvMSP1₁₉GFP^NK65 merozoites (A) or NK65 wild type (WT) merozoites (B) invasion of erythrocytes in vitro with pooled sera taken from naïve or poly (I:C) control mice, or from mice immunized with HIS₆-FliC-PvMSP1₁₉-PADRE + poly (I:C) before challenge (BC) and at day 10 (D10) post-challenge with Pb/PvMSP1₁₉GFP^NK65. Controls are assays with merozoites and erythrocytes with no added serum (Mz no serum), with cytochalasin D (CytD) or with added hyperimmune serum (HI serum). (C) Specific antibody affinity to PvMSP1₁₉ of pooled sera taken from mice immunized with HIS₆-FliC-PvMSP1₁₉-PADRE + poly (I:C) before challenge (BC) and at day 10 (D10) post-challenge with Pb/PvMSP1₁₉GFP^NK65, and of sera taken from naïve or poly (I:C) control mice at day 10 (D10) post-challenge with Pb/PvMSP1₁₉GFP^NK65. (A,B) Unpaired t-test: ns, non-significant; *p < 0.05. (C) One-way ANOVA followed by Tukey's HSD test: ****p < 0.0001.