Figure 6.

Immunizations with HIS₆-FliC-PvMSP1₁₉-PADRE + poly (I:C) protects BALB/c mice from challenge with Pb/PvMSP1₁₉GFP^NK65. BALB/c mice (n = 8) were vaccinated with three immunizing doses of HIS₆-PvMSP1₁₉-PADRE + poly (I:C), HIS₆-FliC-PvMSP1₁₉-PADRE, HIS₆-FliC-PvMSP1₁₉-PADRE + poly (I:C), αDEC-PvMSP1₄₂ + poly (I:C), or poly (I:C) alone as a control (n = 6). HIS₆- is omitted from the name of the recombinant proteins in the figure. (A) Total IgG titers (Log) after the third immunizing dose. Each symbol represents one animal of the indicated groups. (B–E) Mice were challenged i.v. with 5 × 10³ red blood cells infected with the hybrid line Pb/PvMSP1₁₉GFP^NK65 and the parasitemia was followed daily by flow cytometry. Each line represents one mouse of the indicated groups. (F) Mortality curves of the indicated groups. One-way ANOVA followed by Tukey's HSD test: **p < 0.01 and ****p < 0.0001. For mortality curves: Mantel-Cox test.