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Antibody responses in vaccinated mice before challenge and at day 10 post-challenge. Sera titers of total IgG (A), IgG1 (B), IgG2a (C), IgG2b (D), and IgG3 (E) of BALB/c mice in each indicated group (n = 6) before challenge (BC) and at day 10 (D10) post-challenge. Unpaired t-test: ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001.
