Figure 3.

Percentage of viability (A), apoptosis (B) and necrosis (C) of monocyte/lymphocyte stained with supravital exposure to propidium iodide (PI, 50 μg/mL). Cells (1 × 106) were pretreated with LPS and ConA (1 μg/mL and 5 μg/mL, respectively) and with H₂O₂ (4 mM) for 1h, then stained with PI for 30 min at the dark and washed before flow cytometric analysis. The supravital PI exposure on unfixed cells allowed to the simultaneous detection of living (PI negative) apoptotic (PI dim) and necrotic (PI bright) cells.