Figure 2.

IAA8 transcript levels and protein abundance during seed germination. (A) IAA8 transcript level in germinating seeds of Col-0 plants. Seeds of Col-0 plants were germinated on ½ MS medium for the indicated time periods (days). Total RNA was extracted, and RT-qPCR analysis was performed using gene-specific primers; Tubulin2 was used as an internal control. Data are presented as mean values. Error bars represent SE of three biological replicates. Different letters indicate statistically significant differences (P < 0.05). (B) IAA8 protein accumulation during seed germination. Seeds of iaa8-1/IAA8 OX (#6-3) plants were stratified for 4 days in the dark at 4°C and then allowed to germinate on ½ MS medium. Proteins were extracted at the indicated time points (days), and immunoblot analysis was performed using 80 µg of total protein in each well. IAA8 protein was detected using anti-flag antibody. Membranes were stained using Coomassie brilliant blue (CBB). A representative image of three independent experiments is shown.