Table 4.
Associations Between Rheumatoid Factor Positivity and Cytokine Expression After Activation of PBMCs With Immunostimulants
| Association | |||
|---|---|---|---|
| Time | Stimulant | Positive | Negative |
| Visit | CD3/CD28 | Factor 1* . factor 2**, factor 3**, IL-4* IL-5**. IL-17I*, GM-CSD* | … |
| PHA | … | … | |
| PMA/Ion | IL-6* | … | |
| SEA/SEB | Factor 1*,IL-4* IL-5*, IL-13*, GM-CSF* | … | |
| CpG | … | Factor 1**. IL-lβ**, TNF-α**, MIP-lβ** | |
| Medium | … | Factor 1**, factor 3***, G-CSF*, IL-1B*, TNF-α**, IL-6**, IL-17***, GM-cCSF**, MCP-1** | |
| Change between visits | CD3/CD28 | … | IL-5*, IL-17*, GM-CSF* |
| PHA | … | … | |
| PMA/Ion | … | IL-6*, GM-CSF* | |
| SEA/SEB | … | GM-CSDF* | |
| CpG | Factor 1**, factor 2*, factor 3*, G-CSF**, IFN-γ*, IL-lβ**, TNF-α**, MIP-lβ*** GM-CSF*a | … | |
| Medium | Factor 1***, factor 3***, G-CSF*, IFN-γ**, IL-lβ***, TNF-α. IL-6**, IL-17**, GM-CSF**, MCP-1**, MIP-lβ**, IL-2*a, IL-10**a | … | |
| Visit 2 | CD3/CD28 | … | … |
| PHA | … | … | |
| PMA/Ion | … | … | |
| SEA/SEB | … | … | |
| CpG | Factor 1*. factor 3*, G-CSF*, TNF-α* | … | |
| Medium | IFN-γ*, IL-lβ***, TNF-α*, MCP-1*, IL-2*, IL.−10*a | … | |
Abbreviations: CD3/CD28, anti-CD3 and anti-CD28 microbeads; CpG, cytosine-phosphate-guanine oligonucleotide ODN2006; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon; IL, interleukin; MCP, monocyte chemoattractant protein; Medium, RPMI 1640 medium with 10% fetal calf serum; MIP, macrophage inflammatory protein; PBMC, peripheral blood mononuclear cell; PHA, phytohemagglutinin; PMA/Ion, phorbol 12-myristate 13-acetate–ionomycin; SEA/SEB, staphylococcal enterotoxins A and B; TNF, tumor necrosis factor.
Cytokine that was not loaded into a factor at 0.5 or more.
P<.05.
P≤.01.
P≤.001.