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. Author manuscript; available in PMC: 2020 Feb 27.
Published in final edited form as: Nature. 2018 Jul 18;560(7716):102–106. doi: 10.1038/s41586-018-0353-2

Extended Data Figure 9: Assessment of morphologic effects of systemic succinate administration on mouse tissues.

Extended Data Figure 9:

a-f, Representative images of hematoxylin and eosin (a, b, d-f) or Masson’s trichrome (c) staining of indicated tissues harvested from mice following high-fat feeding ± 4 weeks succinate supplementation in drinking water. (a, c, e upper panels 4x magnification, scale bars 1 mm; a, c, e lower panels, b, d, f 40x magnification, scale bars 50 μm). d, Cardiac morphometric analysis ± intervention with 1.5% sodium succinate. Lower panels show representative images of cell width (x40 magnification, scale bars 50 μm). The bar charts indicate quantitative analysis of cardiomyocyte width and length and nuclear diameter (n= 15). g, i.p. glucose tolerance test in mice following high-fat feeding ± 4 weeks succinate supplementation in drinking water quantifying relative changes in glucose upon glucose challenge (n = 9). h, i, mRNA expression of inflammatory (h) and anti-inflammatory (i) markers in the indicated tissues ± intervention with 1.5% sodium succinate in WT and UCP1KO mice (n = 3). g, two-way ANOVA; h, one-way ANOVA; data are mean of biologically independent samples ± s.e.m.