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. 2020 Feb 17;5(7):3258–3270. doi: 10.1021/acsomega.9b03307

Figure 10.

Figure 10

Binding of Ana-4, Ana-5, and indolicidin with plasmid DNA assessed through gel retardation assay. Peptide interaction with plasmid DNA (pBluescriptII SK(+)) was determined via inhibition of DNA migration in 1% (w/v) agarose gel. Different peptide concentrations were incubated with 100 ng of plasmid DNA for 1 h. Lane 1 contains plasmid DNA, and lanes 2–5, 6–9, and 10–13 contain Ana-4, Ana-5, and indolicidin, respectively. The four lanes of each peptide are in decreasing order of peptide concentration, i.e., 100, 50, 25, and 12.5 μM, respectively.