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. Author manuscript; available in PMC: 2020 Feb 27.
Published in final edited form as: Lab Chip. 2019 Oct 2;19(21):3697–3705. doi: 10.1039/c9lc00562e

Fig 3: Diffusion and neutrophil migration in the stacks layers.

Fig 3:

A - A single stacks layer containing collagen-1 and 10kDa FITC-Dextran was placed on top of two other stacks layers, both containing collagen-I only. The FITC-dextran was allowed to diffuse into the bottom two layers for a certain amount of time. B - Results of the diffusion experiment showing average maximum fluorescence for each stacks layer over 90 minutes. C - Results of the double Stacks layer migration experiment showing number of captured neutrophils per layer. D - Results for the ECM continuity experiments showing number of captured neutrophils per ECM condition. All data were quantified from neutrophils collected from 9 stacks layers across 3 independent experiments. Results are consistent across multiple donors. All bars represent mean plus SD. Asterisks represent significance between conditions. * = p < 0.05, **** = p < 0.0001