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. 2020 Feb 27;20:46. doi: 10.1186/s12876-020-01194-2

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Visceral fat findings in the control, MSG, and MSG + FOS mice at 18 weeks. a Histological findings (hematoxylin/eosin staining, 100× magnification, scale bar = 100 μm), b Flow cytometric analysis of ATM. ATM were defined as F4/80⁺ CD11b^high cells. Among the ATM, M1-like macrophages were defined as CD11c⁺ cells. c MFI ratio of CD86 in F4/80⁺ CD11b^high CD11c⁺ cells. ATM, adipose tissue macrophages; FOS, fructo-oligosaccharides; MSG, monosodium glutamate; MFI, mean fluorescence intensity