Table 2.
Fold changes in metabolite levels between infected and uninfected erythrocyte cultures
| Metabolite | FCIDC (SD)a | Pathway | Mass (amu)b | Clusterc |
|---|---|---|---|---|
| 15 metabolites showing the greatest increase | ||||
| Pipecolate | 63.9 (10.1) | Lysine degradation | 130.1 | 5 |
| Nicotinic acid mononucleotide | 33.6 (3.19) | Nicotinate and nicotinamide metabolism | 336.0 | 5 |
| Orotate | 19.8 (2.74) | Pyrimidine metabolism | 155.0 | 1 |
| Phosphoethanolamine | 13.6 (1.14) | Phospholipid metabolism | 140.0 | 5 |
| Glycerol 2-phosphate | 11.0 (1.15) | Glycerolipid metabolism | 171.0 | 5 |
| N1,N12-diacetylspermine | 7.84 (1.73) | Polyamine metabolism | 287.2 | 1 |
| N-acetylserine | 7.71 (0.36) | Glycine, serine, and threonine metabolism | 146.0 | 1 |
| Glycerol | 7.42 (0.98) | Glycerolipid metabolism | 91.0 | 1 |
| Glycerophosphoglycerol | 5.29 (0.23) | Glycerolipid metabolism | 245.0 | 1 |
| Guanine | 4.92 (0.89) | Purine metabolism | 152.1 | 1 |
| Putrescine | 4.87 (0.83) | Polyamine metabolism | 89.1 | 5 |
| 2′-deoxyuridine | 4.80 (0.66) | Pyrimidine metabolism | 227.1 | 5 |
| Nicotinate ribonucleoside | 4.73 (0.67) | Nicotinate and nicotinamide metabolism | 256.1 | 5 |
| 1-stearoyl-GPG (18:0) | 4.69 (0.81) | Lysophospholipid | 511.3 | 5 |
| Mannose-6-phosphate | 4.29 (1.26) | Fructose, mannose, and galactose metabolism | 259.0 | 5 |
| 15 metabolites showing the greatest decrease | ||||
| N6-carboxymethyllysine | 0.37 (0.04) | Advanced glycation end-product | 205.1 | 4 |
| S-lactoylglutathione | 0.37 (0.03) | Glutathione metabolism | 378.1 | 4 |
| Pyrraline | 0.36 (0.06) | Food component/plant | 255.1 | 4 |
| Dihydroxyacetone phosphate | 0.36 (0.03) | Glycolysis, gluconeogenesis, and pyruvate metabolism | 169.0 | 4 |
| 3-methylcytidine | 0.31 (0.04) | Pyrimidine metabolism | 258.1 | 4 |
| 1-linoleoyl-GPC (18:2) | 0.30 (0.04) | Lysophospholipid | 520.3 | 4 |
| 2-phosphoglycerate | 0.29 (0.08) | Glycolysis, gluconeogenesis, and pyruvate metabolism | 185.0 | 4 |
| Ribitol | 0.27 (0.03) | Pentose metabolism | 151.1 | 4 |
| Gamma-glutamylglutamate | 0.27 (0.03) | Gamma-glutamyl amino acid | 277.1 | 4 |
| Isovalerylglycine | 0.26 (0.05) | Leucine, isoleucine, and valine metabolism | 158.1 | 4 |
| 1-stearoyl-GPC (18:0) | 0.25 (0.04) | Lysophospholipid | 524.4 | 4 |
| 1-oleoyl-GPC (18:1) | 0.22 (0.04) | Lysophospholipid | 522.4 | 4 |
| 1-palmitoyl-GPC (16:0) | 0.20 (0.03) | Lysophospholipid | 496.3 | 4 |
| Sedoheptulose-7-phosphate | 0.10 (0.01) | Pentose phosphate pathway | 289.0 | 4 |
| Fructose-6-phosphate | 0.03 (0.00) | Glycolysis, gluconeogenesis, and pyruvate metabolism | 259.0 | 4 |
amu atomic mass unit, GPC glycerophosphocholine, GPG glycerophosphoglycerol, GPI glycerophosphoinositol, GPS glycerophosphoserine, iRBC infected erythrocyte, SD standard deviation, uRBC uninfected erythrocyte
aFold-change (FCIDC) values based on a comparison of the average abundance of a metabolite during the IDC in the iRBC culture relative to that in the uRBC culture
bValues provided by METABOLON©
cCluster in Fig. 2e containing the metabolite