Fig. 6.

Chemo-toxicity up-regulates USP7/hnRNPA1 and promotes miR-522 secretion from CAFs. a-b The effects of cisplatin (a) and paclitaxel (b) on cell viability of CAFs (n = 3). c. The expression of USP7 and hnRNPA1 in CAFs treated with sublethal doses of cisplatin (0.8 μg/ml) and paclitaxel (100 nmol/L) (n = 3). d. Quantitative analysis of (c) (n = 3). e. Chemo-toxicity reduces ubiquitination of hnRNPA1 (n = 3). f. Quantitative analysis of (e) (n = 3). g. Effects of cisplatin and paclitaxel on the expression and secretion of miR-522 (n = 3). h-i. Effects of exosomes derived from chemo-toxicity treated CAFs on ALOX15 expression in GC cells. The ALOX15 protein was determined by WB assay (h) and was quantified by gray analysis (i) (n = 3), and ALOX15 mRNA was checked by qRT-PCR (j) (n = 3). k. Exosomes of chemo-toxicity treated CAFs inhibit erastin induced lipid-ROS production in GC cells (n = 3). l. Exosomes of chemo-toxicity treated CAFs inhibit erastin induced cell death of GC cells (n = 3). m. Exosomes of chemo-toxicity treated CAFs contribute to acquired chemo-resistance of GC cells (n = 3). * indicates p < 0.05 and ** indicates p < 0.01