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. 2020 Feb 13;5(7):3709–3716. doi: 10.1021/acsomega.9b04271

Figure 4.

Figure 4

Effect of NBQX (10 mg/kg, ip) and rapamycin (15 mg/kg, ip) on the phosphorylation of Akt, ERK, mTOR, and GluA1ser845 in the PFC of mice. PFC samples of 4 native mice were collected 1 h after last injection. (A) Western blot analysis of pAkt, pERK, pmTOR, and pGluA1ser845 was performed. Results showed the significantly increased expressions of (B) pAkt, (D) pmTOR, and (E) pGluA1ser845 in mice prefrontal cortex following vanillic acid treatment, but the change of (C) pERK did not achieve statically significant. Note that the increased expressions of (B) pAkt, (D) pmTOR, and (E) pGluA1ser845 resulted from vanillic acid treatment are blocked when mice were pretreated with NBQX. The increased expression of (D) pmTOR resulted from vanillic acid treatment is blocked when mice were pretreated with rapamycin and the increased expressions of (B) pAkt and (E) pGluA1ser845 resulted from vanillic treatment are not blocked. [pAkt, F(3,12) = 7.564, p < 0.01; pERK, F(3,12) = 2.399, p > 0.05; pmTOR, F(3,12) = 4.714, p < 0.05; pGluA1ser845, F(3,12) = 34.337, p < 0.001; n = 4 per group; *p < 0.05; **p < 0.01, ***p < 0.001, comparison to saline + saline group with the Tukey post-hoc analysis]. Total levels of Akt, ERK, mTOR, and GluA1ser845 were not different among the four groups. Values shown are mean ± SEM.