Table 3.

Frequency of homologous integration before and after ligD deletion

Strain (ligD gene)	Obtained transformants	Homologous integrants	Gene-targeting efficiency (%)
HO1 (+)	36	4	11
HO2 (−)	39	38	97

For strain HO1, data were obtained from the integration of the ligD deletion cassette (Fig. 4d). For strain HO2, data were obtained from the integration of the CBH production cassette (Fig. 5a)