Supplement 2.

Effect of propranolol, vagotomy, indomethacin, L-NAME and glibencalamide on bronchoprotection induced by PAR-2-AP

	Baseline (cm)	Control (histamine) (10 µg/kg)	LSIG (l mg/kg)	SLIGRL (l mg/kg)	Propranolol (l mg/kg)	Vagotomy	Indomethacin (5 mg/kg)	L-NAME (30 mg/kg)	Glibenclamide (30 mg/kg)
Change in airway resistance from baseline (%)	4.06±0.48	6.l0±0.69 (cm) +33.49% ±2.25
Change in airway resistance after drug administration compared to control (%)					+20.48±2.99	+11.80±l. 82	+25.82±8.08	+7.55±0.29	+9.94±1.79
Percentage decrease from 1 control min			−3.99*±0.91	−43.94±1.12	−39.83*±8.04	−38.27*±2.22	−34.41*±4.72	−40.24*±2.2	−36.67*±l. 44
Percentage of airway resistance relative to control			96.01	56.06	60.17	61.73	65.59	59.76	63.33
Percentage decrease from 5 control min			−2.99*±0.04	−28.55±3.04	−21.04*±1.12	−26.57±5.20	−25*±1.27	−27.7±1.54	−26.63±4.45
Percentage of airway resistance relative to control			97.01	71.45	75.88	73.43	75	72.3	73.37
Percentage decrease from 10 control mm			−1.49*±0.02	−5.23±l. 60	−7.42*±0.41	−7.36*±0.22	−8.37*±1.94	−6.27±0.87	−6.65±1.53
Percentage of airway resistance relative to control			98.5	94.77	92.58	92.64	91.63	93.73	93.35

Data represent mean±SD of percentage change of airway resistance. *Significant compared to the corresponding SLIGRL value P<0.05. Response to histamine (control) was evaluated 1, 5 and 10 min after SLIGRL administration. SD: Standard deviation