Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 26;21:51. doi: 10.1186/s13059-020-01956-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Inhibition of the in vitro activity of SpCas9 by intact M13 phage and phage-derived G8P_PD peptides. a Dose-dependent inhibition of SpCas9 by intact M13 phage. b Intact M13 phage does not inhibit the in vitro activity of assembled Cas9-sgRNA RNP. c Structural organization of M13 phage major coat protein G8P. d Dose-dependent inhibition of SpCas9 by G8P_PD. e G8P_PD does not inhibit the in vitro activity of assembled Cas9-sgRNA RNP. The above reactions are performed in the absence or presence of 50 nM SpCas9 proteins. The results are shown as mean ± SD (n = 3). Arrows indicate cleavage products