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. 2020 Feb 26;21:51. doi: 10.1186/s13059-020-01956-x

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — G8P_PD prevents Cas9-sgRNA assembly. a Dose-dependent inhibition of Cas9-sgRNA binding by f1 G8P_PD. sgRNA concentration is fixed to 15 μM. b f1 G8P_PD prevents Cas9-sgRNA assembly prior to, but not post, sgRNA addition. Cas9 to sgRNA ratio is fixed to 0.3. The above results are shown as mean ± SD (n = 3)