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Retention of monodispersity and function following steramer conjugation. (A) Autoprocessing of nanoluciferase (Nluc) precursor, Nluc-DHhC. (B) Solution properties and enzymatic activity of native and steramer conjugated Nluc. Unmodified enzyme (top) and ProNAc conjugates of Nluc (asterisk) were separated by size exclusion chromatography (left), then analyzed for enzymatic activity (right). Black triangles in chromatogram indicate excess steramer.
