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Kinetic Parameters of Sterol and Steramer Substrates for Protein−DNA Conjugation Catalyzed by HhC
| substrate | kmax(s−1) (×10−3) | t1/2(min) | Km (μM) | relative proficiency |
|---|---|---|---|---|
| cholesterol | 1.6 | 7.4 | 0.70 | |
| pregnenolone | 1.6 | 7.3 | 11 | 1 |
| preg-methoxime (I) | 2.8 | 4.2 | 5.3 | 3.6 |
| II | 1.2 | 9.3 | 3.0 | 2.8 |
| S-dT10 | 1.8 | 6.4 | 9.2 | 1.3 |
| S-dT30 | 2.0 | 5.8 | 7.1 | 1.9 |
| S-dT39 | 1.1 | 11 | 11 | 0.7 |
