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HPLC-TOF MS analysis of phosphocyclocreatine (5) spiked into V1 product. (A) calibration curve for phosphocyclocreatine-spiked V1 sample; (B) TIC and EIC results for three consecutive V1 injections of 40 μg each. The ion extraction based on the m/z of 224.0431 was within 20 ppm tolerance.
