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. Author manuscript; available in PMC: 2020 Feb 27.
Published in final edited form as: Cell Rep. 2019 Sep 10;28(11):2807–2823.e5. doi: 10.1016/j.celrep.2019.08.014

Figure 2. Spindle Orientation Is an Indicator of Cell Fate.

Figure 2.

(A) Quantification and representative image of twin-spot clones.

(B and C) Quantification (B) and montage (C) of symmetric versus asymmetric outcomes after live imaging of ex vivo intestines. Telophase-cytokinesis was set at 00:00 (hours:minutes). During a symmetric outcome, the dividing cell (−00:30–00:00) forms two daughter cells that remain mCherry− for the duration of the video (08:00). During an asymmetric outcome, the dividing cell (−01:15–00:00) forms two daughter cells. One of the daughter cells remains mCherry−, whereas the other daughter cell becomes mCherry+ (arrow, 06:30). Cell bodies are outlined with green for mCherry− and red for mCherry+. Insets depict 3D reconstruction of the two segregating cell bodies (arrowhead and outlined).

Mean ± SD; n = 133 (young), 112 (short-term refeed), 63 (long-term refeed), 42 (paraquat), and 84 (Ecc15) clones from ≥ 10 flies per condition (A), with spindle orientation quantified from 26 flies (B); N.S., not significant; ***p < 0.001, based on a chi-square test (A) or Mann-Whitney test (B). Red bar, mean (B). Scale bars, 20 μm (A) and 5 μm (C).

See also Figures S2-S4 and Videos S3, S4, S5, S6, S7, and S8.