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. Author manuscript; available in PMC: 2020 Feb 27.
Published in final edited form as: Cell Rep. 2019 Sep 10;28(11):2807–2823.e5. doi: 10.1016/j.celrep.2019.08.014

Figure 6. Regulation of Spindle Orientation during Adaptive Growth Depends on Wdr62 and Kif1a.

Figure 6.

(A) Quantification of spindle orientation after short-term refeeding. Control data for feeding and short-term refeeding were taken from Figure 3B and justified by pooling cells from 18+ flies over 5+ independent experiments.

(B) Percentage of D1+ cells (ISCs) was reduced after Wdr62RNAi or Kif1a overexpression in short-term refed conditions. The number of PH3+ cells in the gut was not affected.

(C) Gut length was decreased after Wdr62RNAi or Kif1a overexpression.

Mean ± SD (B, %D1+ cells, and C) or mean ± SEM (B, #PH3+ cells); n ≥ 9 flies (B, %D1+ cells, and C) and n ≥ 18 flies (B, #PH3+ cells), with spindle orientation quantified from ≥19 flies per condition (A); N.S., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, based on a Kruskal-Wallis test (A–C). Red bar, mean (A). Scale bars, 20 μm (B) and 500 μm (C).