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. Author manuscript; available in PMC: 2020 Feb 27.
Published in final edited form as: J Vis Exp. 2019 Aug 9;(150):10.3791/59836. doi: 10.3791/59836

Figure 3: Immunophenotypic analysis of myeloid lineage cells.

Figure 3:

Single cells were gated based on forward and side scatter. CD34− cells were selected by staining with antibody to CD34 (APC-Cy7). Myeloid lineages in the CD34− population were analyzed with antibodies to CD14 (PE), CD66b (PE-Cy7), CD41 (PerCP-Cy5.5), and CD235a (APC) on day 1 (A) and day 21 (B). Monocytes are CD14+/CD66b−, granulocytes are CD14−/CD66b+, megakaryocytes are CD41+/CD235a− and erythroid cells are CD41−/CD235a+. Representative scatter plots from an experiment are shown. Fractions of monocytes, granulocytes, megakaryocytes, and erythroid cells in the CD34− population on day 1 and day 21 are represented (C). Data presented are averages with standard error from at least 3 experiments. This figure has been modified from Bapat et al.11.