. Author manuscript; available in PMC: 2020 Feb 27.

Published in final edited form as: Stem Cell Res. 2019 Dec 6;42:101673. doi: 10.1016/j.scr.2019.101673

Table 2.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Normal	Fig. 1A
Phenotype	Qualitative analysis: Immunocytochemistry	Positive for SOX2, OCT4, NANOG, SSEA-4	Fig. 1A
	Quantitative analysis: Flow Cytometry (tdTom1, tdTom4)	TRA-1-60 (98.21%, 95.77%); NANOG (99.30%, 92.39%); SSEA-4 (93.11%, 99.46%); tdTomato (99.66%, 99.89%)	Fig. 1B
Genotype	Karyotype (G-banding) and resolution	46XY; Resolution 425–500	Supplementary Fig. S1A
Identity	Microsatellite PCR (mPCR) OR STR analysis	Not performed	N/A
Identity	Microsatellite PCR (mPCR) OR STR analysis	15 loci plus amelogenin (Promega PowerPlex 16) tested, all matched	Available with the authors
Mutation analysis (IF APPLICABLE)	Sequencing	N/A	N/A
Mutation analysis (IF APPLICABLE)	Southern Blot	Monoallelic targeted integration without random integration	Supplementary Fig. S1B
Microbiology and virology	Mycoplasma testing by qPCR	Negative	Supplementary Fig. S1C
Differentiation potential	Teratoma formation	Teratoma formed with three germ layers: Ectoderm, Mesoderm, and Endoderm	Fig. 1C
Donor screening (OPTIONAL)	HIV 1 + 2 Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info (OPTIONAL)	Blood group genotyping	N/A	N/A
Genotype additional info (OPTIONAL)	HLA tissue typing	N/A	N/A