Fig 9. Identification of Leishmania major AlaRS inhibitors.

A) Workflow to identify aminoacylation inhibitors (details described in Methods). B) Representative image of the MNP chemical screen. The spot boxed in red is an example of a predicted inhibitor depicted by the decrease in signal intensity. DMSO positive control (+). C) Three of the four identified inhibitors prevented the accumulation of Ala-tRNA^Ala formation, 1428B was a false-positive result from our preliminary screen. D) The three identified inhibitors perturbed L. major AlaRS activation (black) but had no effect on human AlaRS (gray). The relative amino acid activation is plotted relative to the DMSO control. Error bars indicate the standard deviation of three replicates.