Fig 3. The C-terminal module is sufficient for NMT2 stimulation and protection.

Formation of the myristoyl-peptide by NMT2 was measured in the presence of the indicated proteins over a concentration range of 0.016 to 4μM for 3 min at 37°C. The full data set is presented in S1 Fig. The bar graphs summarize the values obtained at 4μM. Human NMT2 enzyme was added at a concentration of 50nM in the presence of 5μM C₁₄-CoA and in the absence (white bars) or presence of excess competitor C₁₆-CoA (50μM) (filled bars). Control reactions were performed in the absence of the ACBD constructs and of C₁₆-CoA. The data are presented relative to the value obtained in the absence of ACBD (panel A) or in the absence of C₁₆-CoA (panel B). Error bars represent the standard deviations of values obtained from three reactions. **** indicate a p value of <0.0001.