Fig 5. The C-terminal module is sufficient for NMT2 interaction.

NMT2 (10μM) and the linker.ANK (30μM) recombinant protein were dialyzed together at 4°C for 16 hours. As indicated, the proteins were then reacted with the DSS reagent (1.2mM) for 2 hours at 4°C. Reactions were stopped by quenching with a solution of 100mM Tris-HCl pH 7.5. Proteins were separated on denaturing SDS-gradient gel (Any Kd, Bio-Rad). After electrophoresis, gel was stained with Gelcode Blue (Fisher Scientific). The panel shows the image of the gel with the position of NMT2 (48kDa), of linker.ANK (17.5kDa), and of a complex formed after treatment by DSS indicated on the left. The molecular masses of the ladder (Unstained Protein Standard, Broad Range; NewEnglandBiolabs) are shown on the right.