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. 2020 Feb 27;15(2):e0229718. doi: 10.1371/journal.pone.0229718

Fig 6. Acyl-CoA binding to the ACB domain enhances NMT2 activity.

Fig 6

Formation of the myristoyl-peptide by NMT2 (50nM) was measured in the presence of the indicated proteins (4μM) and with 5μM C14-CoA. As indicated, C16-CoA (50μM) and C18:1-CoA (20μM) were added in the reactions. Control reactions were performed in the absence of the ACBD constructs and the data are presented relative to the values obtained in their absence. Error bars represent the standard deviations of values obtained from three reactions. **** indicate a p value of <0.0001. Differences detected with the linker.ANK and ACBD1.linker.ANK forms did not reach statistical significance of p ≤0.05.