Fig 6. CYP3A4 and PXR induction in hepatic progenitors, immature and mature hepatocyte like cells differentiated from human iPSCs.

Human iPSCs-derived hepatocytic cells were exposed to increasing rifampicin concentrations at different differentiation stages: day 10 (hepatic progenitor stage), day 20 (immature hepatocyte stage) and day 25 (mature hepatocyte stage). Following 24 hrs incubation, the changes in the mRNA levels of (A) CYP3A4 and (B) PXR were quantified via quantitative real-time PCR. The data are presented as mean ± SEM, N≥5, * = p < 0.05, ** = p < 0.01.